Immunocytochemical study of YB-1 nuclear distribution in different cell types |
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| Authors: | I.O. Bogolyubova D.N. Lyabin D.S. Bogolyubov L.P. Ovchinnikov |
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| Affiliation: | 1. Laboratory of Cell Morphology, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Avenue, 194064 St. Petersburg, Russia;2. Institute of Protein Research, Russian Academy of Sciences, 4 Institutskaya Street, 142290 Pushchino, Moscow Region, Russia |
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| Abstract: | In the present work we studied the distribution of YB-1 in the nuclei of mouse hepatocytes, early embryos and human skin fibroblasts with the use of light and electron microscopy. To reveal YB-1, we applied rat polyclonal antibody against the C-terminal fragment of YB-1 molecule and rabbit polyclonal antibody against full-length YB-1 molecule. YB-1 distribution patterns varied significantly in different cell types. YB-1 was found to be colocalized with RNA polymerase I in mouse hepatocytes and embryos. Besides, YB-1 was revealed in a population of Cajal bodies in 2-cell mouse embryos but not in other cells studied. |
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| Keywords: | YB-1 Cell nucleus RNA polymerase I Cajal bodies |
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