Noninvasive cross-sectional observation of three-dimensional cell sheet-tissue-fabrication by optical coherence tomography |
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Authors: | Yuji Haraguchi Tatsuya Shimizu Kiminori Mizuuchi Hiroto Kawata Mari Kobayashi Yasushi Hirai Shin-ichi Iwana |
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Affiliation: | 1. Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women?s Medical University, Tokyo 162-8666, Japan;2. Panasonic Healthcare Co., Ltd., 2-38-5 Nishishinbashi, Minato-ku, Tokyo 105-8433, Japan;3. Joint Graduate School of Tokyo Women?s Medical University and Waseda University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan |
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Abstract: | Cell sheet engineering allows investigators/clinicians to prepare cell-dense three-dimensional (3-D) tissues, and various clinical trials with these fabricated tissues have already been performed for regenerating damaged tissues. Cell sheets are easily manipulated and 3-D tissues can be rapidly fabricated by layering the cell sheets. This study used optical coherence tomography (OCT) to noninvasively analyze the following processes: (1) adhesions between layered cell sheets, and (2) the beating and functional interaction of cardiac cell sheet-tissues for fabricating functional thicker 3-D tissues. The tight adhesions and functional couplings between layered cell sheets could be observed cross-sectionally and in real time. Importantly, the noninvasive and cross-sectional analyses of OCT make possible to fabricate 3-D tissues by confirming the adherence and functional couplings between layered cell sheets. OCT technology would contribute to cell sheet engineering and regenerative medicine. |
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Keywords: | Cell adhesion Cell sheet engineering Noninvasive observation Optical coherence tomography Three-dimensional tissue fabrication |
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