On maturation of the bacteriophage lambda chromosome |
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Authors: | Michael Feiss and Thomas Margulies |
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Affiliation: | (1) College of Medicine, Department of Microbiology, University of Iowa, Iowa City, Iowa |
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Abstract: | Summary Repressed lambda chromosomes that possess a duplication of the cohesive end site (cos site) are matured during lytic growth of 80. This result is in contrast to repressed non-duplication lambda chromosomes that are not matured by 80. DNA molecules matured by 80 are unreplicated and lack the duplication: both cos sites are cleaved. These results indicate that in normal lambda development, mature, unit-length chromosomes are generated from a multichromosomal length of lambda DNA by cleavage of two cos sites. |
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