Deinococcus radiodurans DR1088 is a novel RecF‐interacting protein that stimulates single‐stranded DNA annealing |
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| Authors: | Guangzhi Xu Hong Xu Ye Zhao Yuejin Hua |
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| Affiliation: | 1. Agriculture and Food Science School, Zhejiang Agriculture and Forestry University, Zhejiang, ChinaKaiying Cheng and Guangzhi Xu contributed equally to this work.Guangzhi Xu and Yuejin Hua are co‐corresponding authors.;2. Key Laboratory of Chinese Ministry of Agriculture for Nuclear‐Agricultural Sciences, Institute of Nuclear‐Agricultural Sciences, Zhejiang University, Hangzhou, China;3. Key Laboratory of Chinese Ministry of Agriculture for Nuclear‐Agricultural Sciences, Institute of Nuclear‐Agricultural Sciences, Zhejiang University, Hangzhou, ChinaGuangzhi Xu and Yuejin Hua are co‐corresponding authors. |
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| Abstract: | RecF, together with the recombination mediators RecO and RecR, is required in the RecFOR homologous recombination repair pathway in bacteria. In this study, a recF‐dr1088 operon, which is highly conserved in the Deinococcus‐Thermus phylum, was identified in Deinococcus radiodurans. Interaction between DRRecF and DR1088 was confirmed by yeast two‐hybrid and pull‐down assays. DR1088 exhibited some RecO‐like biochemical properties including single/double‐stranded DNA binding activity, ssDNA binding protein (SSB) replacement ability and ssDNA (with or without SSB) annealing activity. However, unlike other recombination proteins, dr1088 is essential for cell viability. These results indicate that DR1088 might play a role in DNA replication and DNA repair processes. |
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