Epitopes recognized by serum anti-α-galactoside antibody are present on brain glycoproteins in man |
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Authors: | Paliakkara L Jaison Vadakkedath M Kannan Mandagini Geetha Padinjaradath S Appukuttan |
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Institution: | (1) Neurochemistry Division, Sree Chitra Tirunal Institute for Medical Sciences and Technology, 695 011 Thiruvananthapuram, India |
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Abstract: | Naturally occurring serum IgG against terminal α-galactoside epitopes (anti-Gal), present exclusively in man, apes and old
world monkeys, was used as probe for these epitopes in human brain. Human brain grey matter soluble glycoproteins enriched
inα galactosyl groups by affinity chromatography on jacalin-sepharose, specifically binds to human anti-Gal in immuno dot
blots. Anti-Gal recognized exclusively the terminal α galactoside epitope in human brain glycoproteins since binding was abolished
by the presence of 1-0-methyl α-D-galactopyranoside as well as by pretreatment of glycoproteins with coffee bean α-galactosidase.
Anti-Gal-peroxidase staining of jacalin-binding human brain glycoproteins in western immuno blots revealed mainly five anti-Gal-binding
polypeptides withM
r
(in kDa) of 94, 108, 180, 210 and 230 respectively. Since the presence of anti-Gal in higher animals accompanies suppression
of the corresponding epitope in most tissues, apparently to maintain immunological balance, possible implications of the above
observation for autoimmunity, tumor metastasis and infection are discussed. |
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Keywords: | Anti-α -galactoside antibody anti-Gal-peroxidase jacalin-binding glycoproteins autoimmunity α -galactoside epitope human brain glycoproteins |
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