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| 杀虫剂对烟粉虱成虫毒力的微量筛选方法 | |
| 引用本文： | 宋晓宇,齐淑华,袁会珠,纪明山,陈小霞.杀虫剂对烟粉虱成虫毒力的微量筛选方法[J].昆虫知识,2006,43(6):877-879. |
| 作者姓名： | 宋晓宇 齐淑华 袁会珠 纪明山 陈小霞 |
| 作者单位： | 1. 中国农业科学院,植物保护研究所,农业部农药化学与应用技术重点开放试验室,北京,100094;沈阳农业大学,植物保护学院,沈阳,110161 2. 中国农业科学院,植物保护研究所,农业部农药化学与应用技术重点开放试验室,北京,100094 3. 沈阳农业大学,植物保护学院,沈阳,110161 |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | 采用直径为18mm的指形管代替常规浸叶法中的培养皿,研究不同寄主植物叶片和琼脂浓度对烟粉虱Bemisia tabaci(Gennadius)成虫自然死亡的影响,并用吡虫啉和啶虫脒为供试药剂对烟粉虱成虫毒力的筛选方法进行比较。结果表明:选用棉花叶片和14g.L-1的琼脂时,指形管内烟粉虱成虫72h自然死亡率最低,只有(5.4±1.5)%;采用此方法就吡虫啉和啶虫脒对烟粉虱成虫的毒力进行测定,测定结果重复性较好,节省药剂用量,无需对烟粉虱成虫麻醉,操作简便,达到微量活体筛选的目标。 |
| 关 键 词： | 烟粉虱成虫 微量活体筛选 毒力测定 |
| 收稿时间： | 12 21 2005 12:00AM |
| 修稿时间： | 2005-12-212006-04-19 |
An in vivo micro-bioassay for toxicity to Bemisia tabaci adult |
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| SONG Xiao-Yu,QI Shu-Hua,YUAN Hui-Zhu,JI Ming-Shan,CHEN Xiao-Xia.An in vivo micro-bioassay for toxicity to Bemisia tabaci adult[J].Entomological Knowledge,2006,43(6):877-879. | |
| Authors: | SONG Xiao-Yu QI Shu-Hua YUAN Hui-Zhu JI Ming-Shan CHEN Xiao-Xia |
| Abstract: | An in vivo micro-bioassay method for insecticidal activity using Bemisia tabaci(Gennadius)adults was studied.The method introduced 18 mm inner diameter test tubes instead of Petri-dishes for leaf-dip method.The influence of different host plant leaves and different agar concentration on B.tabaci adult natural mortality was studied.The results showed that the natural mortality of B.tabaci adult after 72 h was(5.4±1.5)% by the micro-bioassay method with 14 g·L-1 agar concentration.The toxicities of imidacloprid and acetamiprid to B.tabaci using this micro-bioassay method were well repeatable and could save pesticide usage.It showed that this method was suitable for insecticide bioassay against B.tabaci. |
| Keywords: | Bemisia tabaci adult micro-bioassay toxicity |
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