Trans-stimulation and driving forces for GSH transport in sinusoidal membrane vesicles from rat liver |
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| Authors: | T Y Aw M Ookhtens J F Kuhlenkamp N Kaplowitz |
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| Institution: | 1. Department of Mechanical Engineering, Nazarbayev University, Astana, Kazakhstan;2. School of Engineering and ICT, University of Tasmania, Hobart, Australia |
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| Abstract: | Sinusoidal membrane vesicles from rat liver were employed to study the characteristics of GSH transport. Saturable concentration dependent uptake was best described by the sum of a high and low Km transport. Preloading with GSH markedly stimulated the initial uptake of GSH. GSH transport was electrogenic; uptake was enhanced by an inwardly directed K+ gradient which could be blocked by the K+-channel blocker, Ba2+. The other cations such as Na+, Li+ were poor substitutes for K+. These results therefore show that net GSH transport involves movement of K+. |
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