| 添加有机酸对Clostridium acetobutylicum合成丙酮和丁醇的影响 |
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| 引用本文： | 唐波,余晓斌,李灵巧.添加有机酸对Clostridium acetobutylicum合成丙酮和丁醇的影响[J].生物加工过程,2008,6(3):24-28. |
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| 作者姓名： | 唐波 余晓斌 李灵巧 |
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| 作者单位： | 江南大学,生物工程学院,工业生物技术教育部重点实验室,无锡,214122 |
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| 基金项目： | 长江学者与创新团队发展计划 |
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| 摘 要： | 为提高丙酮-丁醇梭菌厌氧发酵生产丙酮和丁醇的能力,在发酵过程中添加有机酸（乙酸和丁酸）,考察其对菌体生长、溶剂合成影响。实验表明：当添加1.5 g/L乙酸时能够促进菌体的生长,促进丙酮的合成,在600 nm处的最大OD值比参照值高出18.4%,丙酮的最终质量分数提高了21.05%,但不能促进丁醇的合成;当添加1.0g/L丁酸时能够促进菌体生长,促进丁醇的合成,在600 nm处的最大OD比参照值高22.29%,丁醇的最终质量分数比对照组提高了24.32%,但不能促进丙酮的合成。
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| 关 键 词： | 丙酮 丁醇 丙酮丁醇梭菌 发酵 有机酸 |
| 文章编号： | 1672-3678(2008)03-0024-05 |
| 修稿时间： | 2007年8月14日 |
Effect of acetic and butyric acids on fermentation production of acetone-butanol by Clostridium acetobutylicum |
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| TANG Bo,YU Xiao-bin,LI Ling-qiao.Effect of acetic and butyric acids on fermentation production of acetone-butanol by Clostridium acetobutylicum[J].Chinese Journal of Bioprocess Engineering,2008,6(3):24-28. |
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| Authors: | TANG Bo YU Xiao-bin LI Ling-qiao |
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| Institution: | (The Key Laboratory of Industrial Biotechnology of Ministry of Education, College of Biotechnology, Jiangnan University, Wuxi 214122, China) |
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| Abstract: | To improve acetone-butanol production efficiency by Clostridium acetobutylicum, acetic and butyric acid were added to the fermentation broth to investigate the effect on cell growth and production of acetone-butanoL Addition of 1.5 g/L acetic acid increased the cell growth and enhanced acetone production, the final concentration of acetone was 21.05%, and the butanol production was not improved. Addition of 1.0 g/L butyric acid increased the cell growth and enhanced butanol production, the final concentration of butanol was 24. 32%, while the acetone production was not improved. |
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| Keywords: | acetone butanol Clostridium acetobutylium fermentation organic acid |
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