Molecular polymorphism and phenotypic variation in Aspergillus carbonarius |
| |
Authors: | Ferenc Kevei Zsuzsanna Hamari János Varga Zofia Kozakiewicz James H Croft |
| |
Institution: | (1) Department of Microbiology, Attila József University, P.O. Box 533, H-6701 Szeged, Hungary;(2) International Mycological Institute, Bakeham Lane Englefield Green, TW20 9TY Egham, Surrey, UK;(3) School of Biological Sciences, University of Birmingham, P.O. Box 363, B15 2TT Birmingham, United Kingdom |
| |
Abstract: | Thirteen collection strains and field isolates of Aspergillus carbonarius were examined by using various genotypic and phenotypic approaches. Restriction fragment length polymorphism analysis of the ribosomal RNA gene cluster and the mitochondrial DNA of the strains revealed only slight variations, except for one field isolate (IN7), which exhibited completely different ribosomal RNA gene cluster and mitochondrial DNA patterns. The mitochondrial DNAs of these strains were found to be much larger (45 to 57 kb) than those found earlier in the A. niger aggregate. Strain-specific characters could be detected by the random amplified polymorphic DNA technique. Isoenzyme analysis and examination of carbon source utilisation patterns of the strains also revealed some intraspecific variability, though much smaller than that observed by using DNA-based techniques. The dendrograms constructed based on genotypic and phenotypic data suggest that strain IN7 might represent a new subspecies of A. carbonarius.Abbreviations kb
kilobase pair
- mtDNA
mitochondrial DNA
- RAPD
random amplified polymorphic DNA
- rDNA
ribosomal RNA gene cluster
- RFLP
restriction fragment length polymorphisms |
| |
Keywords: | Aspergillus carbonarius isoenzyme mtDNA rDNA RFLPs RAPD |
本文献已被 SpringerLink 等数据库收录! |
|