Kinetic and metabolic analysis of mouse embryonic stem cell expansion under serum-free conditions |
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Authors: | Tiago G Fernandes Ana M Fernandes-Platzgummer Cláudia Lobato da Silva Maria Margarida Diogo and Joaquim M S Cabral |
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Institution: | (1) Institute for Biotechnology and Bioengineering (IBB), Centre for Biological and Chemical Engineering, Instituto Superior T?cnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal; |
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Abstract: | There is a need for a deeper understanding of the biochemical events affecting embryonic stem (ES) cell culture by analyzing
the expansion of mouse ES cells in terms of both cell growth and metabolic kinetics. The influence of the initial cell density
on cell expansion was assessed. Concomitantly, the biochemical profile of the culture was evaluated, which allowed measuring
the consumption of important substrates, such as glucose and glutamine, and the production of metabolic byproducts, like lactate.
The results suggest a more efficient cell metabolism in serum-free conditions and a preferential use of glutaminolysis as
an energy source during cell expansion at low seeding densities. This work contributes to the development of fully-controlled
bioprocesses to produce relevant numbers of ES cells for cell therapies and high-throughput drug screening. |
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