Newly Isolated Bacillus gibsonii S-2 Capable of using Sugar Beet Pulp for Alkaline Pectinase Production |
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Authors: | Zuming Li Zhihui Bai Baoguo Zhang Huijun Xie Qing Hu Chunbo Hao Wentong Xue Hongxun Zhang |
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Institution: | (1) Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 100085, Beijing, China;(2) College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China;(3) School of Natural and Built Environments, University of South Australia, 5095 Mawson Lakes, SA, Australia |
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Abstract: | Summary A Bacillus strain capable of growing under highly alkaline conditions was isolated from a sample of alkaline soil. The isolate was a
Gram-positive, spore-forming, aerobic, alkaliphilic bacterium and was designated as strain S-2. Growth of the strain was observed
in the pH range of 7–12 and temperature range of 4–40 °C. Sequence analysis of the 16S rDNA of the strain revealed more than
99% homology with the strains of Bacillus gibsonii. The S-2 strain was confirmed as B. gibsonii by comparing its physiological and biochemical characteristics with the B. gibsonii DSM 8722 strain. The S-2 strain could use sugar beet pulp as the carbon source as well as the pectinase inducer to produce
extracellular alkaline pectinase by solid-state fermentation. The maximum polygalacturonase yield of 3600 U/g dry sugar beet
pulp was obtained at 35 °C after 48 h of incubation. |
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Keywords: | Alkaline pectinase Bacillus gibsonii phenotypic characterization 16S rDNA solid-state fermentation |
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