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Evaluation of molecular assays for identification Campylobacter fetus species and subspecies and development of a C. fetus specific real-time PCR assay |
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| Authors: | Linda van der Graaf-van Bloois Marcel A.P. van Bergen Fimme J. van der Wal Albert G. de Boer Birgitta Duim Tracy Schmidt Jaap A. Wagenaar |
| Affiliation: | 1. Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80165, 3508 TD Utrecht, The Netherlands;2. Central Veterinary Institute of Wageningen UR, P.O. Box 65, 8200 AB Lelystad, The Netherlands;3. WHO Collaborating Center for Campylobacter and OIE Reference Laboratory for Campylobacteriosis, The Netherlands;4. Allerton Provincial Veterinary Laboratory, Private Bag X2, Cascades, 3202, South Africa |
| Abstract: | Phenotypic differentiation between Campylobacter fetus (C. fetus) subspecies fetus and C. fetus subspecies venerealis is hampered by poor reliability and reproducibility of biochemical assays. AFLP (amplified fragment length polymorphism) and MLST (multilocus sequence typing) are the molecular standards for C. fetus subspecies identification, but these methods are laborious and expensive. Several PCR assays for C. fetus subspecies identification have been described, but a reliable comparison of these assays is lacking. |
| Keywords: | AFLP Bovine genital campylobacteriosis Campylobacter fetus MLST Real-time PCR Subspecies identification |
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