High-density,homogeneous endospore monolayer deposition on test surfaces |
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Authors: | Aaron C. Noell Arin R. GreenwoodChristine M. Lee Adrian Ponce |
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Affiliation: | Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Drive, Pasadena, CA 91109, USA |
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Abstract: | Bacillus subtilis spores were deposited in high-density single layers on metal, glass, and polymer substrates using vacuum filtration followed by a wetted filter transfer step. Quantitative analysis of spore transfer was performed using culture-based and germinability assays, and spore distributions were observed with electron microscopy. |
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Keywords: | B. subtilis Spores Vacuum filtration Inactivation Deposition |
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