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High-density,homogeneous endospore monolayer deposition on test surfaces
Authors:Aaron C. Noell  Arin R. GreenwoodChristine M. Lee  Adrian Ponce
Affiliation:Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Drive, Pasadena, CA 91109, USA
Abstract:Bacillus subtilis spores were deposited in high-density single layers on metal, glass, and polymer substrates using vacuum filtration followed by a wetted filter transfer step. Quantitative analysis of spore transfer was performed using culture-based and germinability assays, and spore distributions were observed with electron microscopy.
Keywords:B. subtilis   Spores   Vacuum filtration   Inactivation   Deposition
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