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Propidium monoazide does not fully inhibit the detection of dead Campylobacter on broiler chicken carcasses by qPCR |
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| Authors: | Ewa Pacholewicz Arno Swart Len JA Lipman Jaap A Wagenaar Arie H Havelaar Birgitta Duim |
| Institution: | 1. Division Veterinary Public Health, Institute for Risk Assessment Sciences, Utrecht University, Utrecht, 3508 TD, The Netherlands;2. Research & Development, MEYN Food Processing Technology B.V., Oostzaan, 1511 MA, The Netherlands;3. Laboratory for Zoonoses and Environmental Microbiology, National Institute for Public Health and the Environment, Bilthoven, 3720 BA, The Netherlands;4. Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, 3508 TD, The Netherlands;5. Central Veterinary Institute of Wageningen UR, Lelystad, The Netherlands;6. WHO-Collaborating Center for Campylobacter/OIE Reference Laboratory for Campylobacteriosis, Utrecht/Lelystad, The Netherlands |
| Abstract: | A real time quantitative PCR combined with propidium monoazide (PMA) treatment of samples was implemented to quantify live C. jejuni, C. coli and C. lari on broiler chicken carcasses at selected processing steps in the slaughterhouse. The samples were enumerated by culture for comparison. The Campylobacter counts determined with the PMA-qPCR and the culture method were not concordant. We conclude that the qPCR combined with PMA treatment of the samples did not fully reduce the signal from dead cells. |
| Keywords: | Campylobacter qPCR PMA Broiler |
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