A new,rapid and sensitive bioluminescence assay for drug screening on Leishmania |
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| Authors: | Lucie Paloque Nicolas Vidal Magali Casanova Aurélien Dumètre Pierre Verhaeghe Daniel Parzy Nadine Azas |
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| Institution: | 1. Infections Parasitaires, Transmission, Physiopathologie et Thérapeutique, UMR MD3, Faculté de Pharmacie, Aix-Marseille Université, 27 Boulevard Jean Moulin, CS 30064, 13385 Marseille Cedex 05, France;2. Institut de Chimie Radicalaire, UMR CNRS 7273, Faculté des Sciences de St Jérôme, Aix Marseille Université, 13397 Marseille Cedex 20, France;3. Equipe Pharmacochimie Radicalaire, Institut de Chimie Radicalaire, UMR CNRS 7273, Faculté de Pharmacie, Aix-Marseille Université, 27 Boulevard Jean Moulin, CS 30064, 13385 Marseille Cedex 05, France |
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| Abstract: | We validated a new method, based on luciferine/luciferase bioluminescence, for drug screening on promastigotes of different Leishmania species. Results obtained with this new, rapid, reproducible, and reliable method are in good accordance with results obtained by the conventional MTT assay. This bioluminescence assay has a lower detection limit. |
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| Keywords: | Leishmania Luminescence Screening Assay validation Linearity Accuracy |
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