Whole genome amplification of the obligate intracellular pathogen Coxiella burnetii using multiple displacement amplification |
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Authors: | Sanjay Kumar Shefali Raj Gangoliya Mustapha Berri Annie Rodolakis Syed Imteyaz Alam |
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Institution: | 1. Biotechnology Division, Defence Research & Development Establishment, Gwalior 474002, India;2. INRA, UMR1282 ISP, Nouzilly, France |
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Abstract: | This study demonstrates that whole genome multiple displacement amplification (MDA) is a promising technique for downstream genomic analysis of fastidious obligate intracellular pathogens such as Coxiella burnetii. The MDA technology can help in obtaining sufficient genetic material from highly infectious agent and thus minimizing repeated culturing and associated biohazard. |
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Keywords: | Coxiella burnetii Whole genome amplification Multiple displacement amplification |
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