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Use of Strep-tag II for rapid detection and purification of Mycobacterium tuberculosis recombinant antigens secreted by Streptomyces lividans |
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| Authors: | Julio C Ayala Elsa Pimienta Caridad Rodríguez Jozef Anné Carlos Vallín María T Milanés Emmanuel King-Batsios Kris Huygen Lieve Van Mellaert |
| Institution: | 1. Institute of Pharmacy and Food, University of Havana, Havana, Cuba;2. Laboratory of Molecular Bacteriology, Rega Institute for Biomedical Research, Katholieke Universiteit Leuven, Leuven, Belgium;3. National Hospital of Pneumology “Benéfico Jurídico”, Havana, Cuba;4. Service of Immunology, Scientific Institute of Public Health, Brussels, Belgium |
| Abstract: | |
| Keywords: | Streptomyces lividans Mycobacterium tuberculosis Secretory protein production Purification Strep-tag® II Affinity chromatography |
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