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Simultaneous detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in soil using immunomagnetic separation and direct fluorescent antibody staining
Authors:Ezra Orlofsky  Osnat Gillor  Ann Melli  Woutrina Miller  Stefan Wuertz  Nirit Bernstein  Karen Shapiro
Institution:1. Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben-Gurion University, Midreshet Ben Gurion, 84990 Israel;2. Department of Pathology, Microbiology, and Immunology, University of California, One Shields Avenue, Davis, CA 95616, USA;3. Department of Civil and Environmental Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA;4. Singapore Centre on Environmental Life Sciences Engineering, School of Biological Sciences SBS-B1N-27, and School of Civil and Environmental Engineering, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore;5. Institute of Soil Water and Environmental Sciences, Volcani Center, POB 6, Bet-Dagan, 50–250, Israel
Abstract:An improved approach for simultaneous detection of Cryptosporidium parvum and Giardia lamblia (oo)cysts in soil is described. Recoveries > 70% were obtained for concentrations > 55 and 21 (oo)cysts g −1 for C. parvum and G. lamblia, respectively. The limits of detection were determined to be < 5 (oo)cysts g −1 soil.
Keywords:Cryptosporidium parvum  Detection  Giardia lamblia  Immunomagnetic separation  Recovery  Soil contamination
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