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Rapid assay for detecting gyrA and parC mutations associated with fluoroquinolone resistance in Enterobacteriaceae |
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| Authors: | Ryuichi Nakano Ryoichi Okamoto Akiyo Nakano Noriyuki Nagano Michiko Abe Shigeru Tansho-Nagakawa Tsuneyuki Ubagai Takane Kikuchi-Ueda Osamu Koshio Hirotoshi Kikuchi Yasuo Ono |
| Institution: | 1. Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173–8605, Japan;2. Department of Microbiology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252–0374, Japan;3. Medical Microbiology Laboratory, Funabashi Municipal Medical Center, 1-21-1 Kanasugi, Funabashi, Chiba 273–8588, Japan;4. Department of Medical Laboratory Sciences, School of Allied Health Sciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252–0373, Japan |
| Abstract: | We developed a PCR-RFLP assay to detect mutations in the quinolone-resistance determining regions of gyrA and parC associated with fluoroquinolone resistance in Enterobacteriaceae. The assay detected mutations associated with reduced susceptibility to fluoroquinolones and may therefore serve as a specific, rapid, inexpensive, and simple testing alternative. |
| Keywords: | Fluoroquinolones Quinolone resistance-determining regions Enterobacteriaceae PCR-restriction fragment length polymorphism |
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