Interference of ferric ions with ferrous iron quantification using the ferrozine assay |
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| Authors: | Jeongdae Im,Jaejin Lee,Frank E. Lö ffler |
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| Affiliation: | 1. Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA;2. Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN 37996, USA;3. Department of Civil and Environmental Engineering, University of Tennessee, Knoxville, TN 37996, USA;4. University of Tennessee and Oak Ridge National Laboratory (UT–ORNL) Joint Institute for Biological Sciences (JIBS) and Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA |
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| Abstract: | The ferrozine assay is a widely used colorimetric method for determining soluble iron concentrations. We provide evidence for a heretofore unrecognized interference of ferric ions (Fe3 +) on ferrous iron (Fe2 +) measurements performed in the dark. Fe3 + concentrations affected the absorbance measurements, which linearly increased with incubation time. |
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| Keywords: | Ferrozine Ferrous Ferric Interference |
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