外源一氧化氮对盐胁迫下黄瓜幼苗生长和渗透调节物质含量的影响

采用水培法,研究了外源一氧化氮(NO)对黄瓜幼苗生长和渗透调节物质含量的影响。结果表明:正常生长条件下添加外源NO能促进黄瓜幼苗生长,而添加NO信号传递途径关键酶——鸟苷酸环化酶抑制剂亚甲基蓝(MB-1)显著抑制了黄瓜幼苗的生长;盐胁迫条件下,添加外源NO明显缓解了盐胁迫对黄瓜幼苗生长的抑制,与单独盐处理比较,株高、茎粗、鲜质量、干质量显著增加,渗透调节物质如可溶性糖、可溶性蛋白和脯氨酸含量明显提高,而MB-1能够不同程度地消除NO的这些调节作用;NO对盐胁迫下黄瓜幼苗生长影响大于正常生长条件下黄瓜幼苗,NO的作用可能是通过鸟苷酸环化酶介导的。

Effects of exogenous nitric oxide on Cucumis sativus seedlings growth and osmoatic adjustment substances contents under NaCl stress

The study with water culture showed that under normal conditions,exogenous nitric oxide could promote the growth of Cucumis sativus seedlings,while methylene blue(MB-1),an inhibitor of guanylate cyclases,could inhibit the growth markedly.Under NaCl stress,nitric oxide alleviated the NaCl injury markedly and increased the plant height,stem thickness,dry and fresh biomass,and soluble sugar and soluble protein contents of the seedlings significantly,while MB-1 blocked these effects to some extent.The effects of nitric oxide on C.sativus seedlings growth were more prominent under NaCl stress than under normal conditions,suggesting that the effects could be induced by guanylate cyclases.