Reduced toxicity of amphotericin B methyl ester (AME) vs. amphotericin B and fungizone in tissue culture |
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Authors: | Paul B Fisher Neil I Goldstein Vernon Bryson Carl P Schaffner |
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Institution: | (1) Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, 10461 Bronx, N.Y.;(2) Waksman Institute of Microbiology, Rutgers University, 08903 New Brunswick, New Jersey |
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Abstract: | Summary The comparative toxicities of amphotericin B methyl ester (AME), the parent antibiotic amphotericin B (AB), and the deoxycholate
solubilized complex of AB, Fungizone2 (FZ), toward five cell lines has been determined as measured by early membrane damage (51Cr release), 24 hr survival, 72 hr viability, and growth rate. Cells used were of turtle (TH-1), marsupial (PT K2), human
MA 160), rabbit (RK-13) and hamster (BHK-21) origin. AME: (a) caused less membrane damage at 1 hr than AB or FZ; (b) was less
toxic than AB or FZ as indicated by 24 hr cell survival and 72 hr cell viability; and (c) was required in higher levels than
AB or FZ to reduce the growth rate of all five cell lines. Spectrophotometric analysis of residual polyene levels indicated
that AME had good stability in tissue culture medium. Previous studies have indicated that AME has the same in vitro antifungal
activity as the parent antibiotic AB (1, 2). These findings suggest that AME may prove to be superior to AB and FZ for use
as an antifungal agent in tissue culture systems.
FungizoneR. Trade mark. E. R. Squibb and Sons.
This investigation was supported in part by Contract NIH 69-2161, NIH Grant No. AI-02095 and NIH Training Grant No. GM 507
from the National Institute of General Medical Sciences. |
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Keywords: | amphotericin B methyl ester (AME) amphotericin B (AB) Fungizone (FZ) toxicity polyene antibiotic |
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