Induction of multiple shoots from leaf segments, in vitro-flowering and fruiting of a dwarf tomato (Lycopersicon esculentum) |
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Authors: | Rao Kokkirala Venugopal Kiranmayee Kasula Pavan Umate Sree Telakalapalli Jaya Rao Alleni V Sadanandam Abbagani |
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Affiliation: | Department of Botany, Plant Biotechnology Research Unit, Kakatiya University, Warangal 506 009, India. |
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Abstract: | Multiple shoots were induced from leaf explants of Lycopersicon esculentum cultivar MicroTom, within 20-25d, on MS medium supplemented with 8.9 microM benzylaminopurine (BAP)+1.14 microM indole-3-acetic acid (IAA). For rooting, elongated microshoots were excised and transferred onto MS medium supplemented with 4.9 microM indole-3-butyric acid (IBA). Well-developed roots and flower raceme were obtained on d 7 and 13, respectively, upon transfer of the microshoots onto rooting medium. The flowers self-fertilized in vitro and produced mature fruits in additional 15-17d of culture. |
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Keywords: | Auxin In vitro flower induction Microshoots Tomato cv. MicroTom |
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