Analysis of nucleotide pools during protease production with Bacillus licheniformis |
| |
Authors: | Gabriele Bierbaum Ulrich Eberhard Giesecke Christian Wandrey |
| |
Institution: | (1) Institut für Biotechnologie 2 des Forschungszentrums Jülich, Postfach 19 13, W-5170 Jülich, Federal Republic of Germany;(2) Present address: Institut für Medizinische Mikrobiologie und Immunologie der Universität, Sigmund-Freud-Strasse 25, W-5300 Bonn 1, Federal Republic of Germany;(3) Present address: Boehringer Mannheim GmbH, Nonnenwald 2, W-8122 Penzberg, Federal Republic of Germany |
| |
Abstract: | Summary During development of a fed-batch procedure for protease production with Bacillus licheniformis the nucleotide pools of the culture were assayed. Transitions between different growth phases or different nutrient limitations could easily be discerned by alterations in the nucleotide pool. As already described for B. subtilis, induction of sporulation was marked by a drop in the guanosine triphosphate (GTP) pool of the cells, which always preceded protease production. This was also valid for closed-loop controlled fed-batch processes in which the concentration of ammonium, which repressed protease biosynthesis, was kept constant at low levels. A marked decrease in the GTP content of the cells, e.g. after addition of mycophenolic acid during the exponential phase, increased protease formation during the stationary phase. During protease production the energy charge was lower (0.6–0.8) than during the exponential and early stationary phases (0.8–0.95) although very low energy charges (<0.5) did not support protease formation.
Offprint requests to: G. Bierbaum |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|