Purification and characterization of an endonuclease (E.C. 3.1.30.1) from Streptomyces tendae |
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| Authors: | P Engel A H Ullah |
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| Affiliation: | Southern Regional Research Laboratory, ARS, New Orleans, Louisiana 70124. |
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| Abstract: | A single-strand-specific endonuclease which converted negatively supercoiled DNA to open-circular and linear DNA was purified to homogeneity with Hb-Sepharose 4B, DEAE Trisacryl M, HA-Ultrogel and PBE-94 chromatofocusing from extracts of Streptomyces tendae ATCC 31160. Bio-Gel P-200 chromatography and electrophoresis in SDS-PAGE indicated the native protein was a monomer with a molecular weight of approximately 40-kDa. This enzyme did not hydrolyze double-stranded linear DNA but digested RNA and circular single-strand DNA. Sequence specificity for nicking of negatively supercoiled DNA was not detected. |
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