Isolation of cytochrome bc
1 complexes from the photosynthetic bacteria Rhodopseudomonas viridis and Rhodospirillum rubrum |
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| Authors: | R Max Wynn Dale F Gaul Won-Ki Choi Robert W Shaw David B Knaff |
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| Institution: | (1) Department of Chemistry and Biochemistry, Texas Tech University, 79409 Lubbock, Texas, USA;(2) Present address: Department of Biochemistry, University of Wisconsin, 53706 Madison, Wisconsin, USA;(3) Present address: Department of Chemistry, Chonnam National University, 500 Kwangju, Chonnam, Republic of Korea;(4) Department of Chemistry and Biochemistry, Texas Tech University, 79409 Lubbock, Texas, USA |
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| Abstract: | Cytochrome bc
1 complexes have been isolated from wild type Rhodopseudomonas viridis and Rhodospirillum rubrum and purified by affinity chromatography on cytochrome c-Sepharose 4B. Both complexes are largely free of bacteriochlorophyll and carotenoids and contain cytochromes b and c
1 in a 2:1 molar ratio. For the Rps. viridis complex, evidence has been obtained for two spectrally distinct b-cytochromes. The R. rubrum complex contains a Rieske iron-sulfur protein (present in approximately 1:1 molar ratio to cytochrome c
1) and catalyzes an antimycin A- and myxothiazol-sensitive electron transfer from duroquinol to equine cytochrome c or R. rubrum cytochrome c
2. Although an attempt to prepare a cytochrome bc
1 complex from the gliding green bacterium Chloroflexus aurantiacus was not successful, membranes isolated from phototrophically grown Cfl. aurantiacus were shown to contain a Rieske iron-sulfur protein and protoheme (the prosthetic group of b-type cytochromes).Dedicated to Prof. L.N.M. Duysens on the occasion of his retirement. |
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| Keywords: | cytochrome bc
1 complex Rieske iron-sulfur protein photosynthetic bacteria |
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