Quantification of 3,4-methylenedioxymetamphetamine and its metabolites in plasma and urine by gas chromatography with nitrogen–phosphorus detection |
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Authors: | Jordi Ortuo Nieves Pizarro Magí Farr Marta Mas Jordi Segura Jordi Camí Rudolf Brenneisen Rafael de la Torre |
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Institution: | Jordi Ortuño, Nieves Pizarro, Magí Farré, Marta Mas, Jordi Segura, Jordi Camí, Rudolf Brenneisen,Rafael de la Torre, |
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Abstract: | A gas chromatographic method with nitrogen–phosphorus detection involving a solid–liquid extraction phase was developed and validated for the simultaneous quantification of 3,4-methylenedioxymethamphetamine (MDMA) and 3,4-methylenedioxyamphetamine (MDA) in plasma. A modification of this method was validated for the analysis of MDMA, MDA, 4-hydroxy-3-methoxymethamphetamine (HMMA) and, 4-hydroxy-3-methoxyamphetamine (HMA) in urine. Under the analytical conditions described, the limits of detection in plasma and urine were less than 1.6 μg/l and 47 μg/l, respectively, for all the compounds studied. Good linearity was observed in the concentration range evaluated in plasma (5–400 μg/l) and urine (100–2000 μg/l) for all compounds tested. The recoveries obtained from plasma were 85.1% and 91.6% for MDMA and MDA, respectively. Urine recoveries were higher than 90% for MDMA and MDA, 74% for HMMA, and 64% for HMA. Methods have been successfully used in the assessment of plasma and urine concentrations of MDMA and its main metabolites in samples from clinical studies in healthy volunteers. |
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Keywords: | 3 4-Methylenedioxymethamphetamine 3 4-Methylenedioxyamphetamine |
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