Control of suppressor cell activity: autoanti-idiotype B cells produced by painting with picryl chloride inhibit the T-suppressor cell which blocks the efferent stage of contact sensitivity |
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Authors: | G L Asherson M Zembala S C Gautam M C Watkins |
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Affiliation: | 1. Division of Immunological Medicine, Clinical Research Centre, Harrow, United Kingdom;2. Division of Clinical Immunology, Institute of Paediatrics, Copernicus Medical School, Cracow, Poland |
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Abstract: | This paper describes a B “suppressor of suppressor” cell which blocks the production or action of the T-suppressor cell, Ts-eff (cs), which acts at the efferent stage of the contact sensitivity reaction. Ts-eff (cs) occur in mice 7 days after injecting picrylsulfonic acid (PSA) and are assayed by their ability to block the passive transfer of contact sensitivity in a 24-hr experiment. These Ts-eff (cs) cannot be demonstrated in mice painted with picryl chloride and injected with PSA 8 days later. In fact, 8 days after painting mice contain B cells which prevent the appearance of Ts-eff (cs) following the injection of PSA. Moreover, the serum of mice 12 days after painting contains antibody which inactivates Ts-eff (cs). This antibody is anti-idiotypic as shown by its absorption to and elution from insolubilized mouse anti-picryl antibody and the lack of effect of absorption with insolubilized picryl groups. The antibody belongs to the IgG2a class and requires an intact Fc moiety for its action. |
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Keywords: | LN lymph node MEM minimum essential medium Ox oxazolone i.e., 4-ethoxymethylene-2-phenyloxazolone PBS Dulbecco's phosphate-buffered saline PCL picryl chloride Pic picryl PSA picrylsulfonic acid (trinitrobenzenesulfonic acid) Ts-eff (cs) the T-suppressor cell which acts at the efferent stage of the contact sensitivity reaction SPL spleen |
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