<Emphasis Type="Italic">CAPN1</Emphasis> markers in three Argentinean cattle breeds: report of a new InDel polymorphism within intron 17 |
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Authors: | Email author" target="_blank">P?P?IglesiasEmail author M?E?Caffaro A?F?Amadio A?Arias Ma?otti Email author" target="_blank">M?A?PoliEmail author |
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Institution: | 1.Instituto de Genética “Ewald A. Favret”, CICVyA-INTA,Buenos Aires,Argentina;2.CONICET, EEA Rafaela—INTA,Rafaela,Argentina;3.EEA Corrientes—INTA,Corrientes,Argentina |
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Abstract: | In this study, the genotype distribution and allelic frequencies of CAPN1 (Calcium activated neutral protease) single nucleotide polymorphisms (SNPs) were analyzed taking advantage of the different
genetic backgrounds provided by Hereford, Brahman and Braford cattle. We report a new insertion/deletion (InDel) polymorphism,
consisting of a change of seven nucleotides for only one nucleotide (TCTGGGT → C) within intron 17 of the CAPN1 gene. The segregation pattern of this polymorphism was analyzed together with the markers CAPN316, CAPN530 and CAPN4751 already
described. The allele distribution of CAPN1 markers in the Braford crossbreed (3/8 Brahman 5/8 Hereford) is described for the first time. Four assays of allelic discrimination
were designed: the tetra primer ARMS-PCR technique for genotyping the new InDel and the CAPN4751 marker, and a PCR-RFLP method
for genotyping the markers CAPN316 and CAPN530. The genotypic and minor allele frequencies (MAFs) obtained showed that the
InDel polymorphism does not provide redundant information to that already provided by the other CAPN1 markers and segregates differently between breeds, being a common SNP (MAF ≥ 0.05) in the herds with a high percentage of
Bos indicus background. The high percentage of heterozygous individuals found in the Braford crossbreed for the markers assessed reveals
enough genetic variation that could help to solve the tenderness problem of tropical-adapted cattle. |
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