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Production and regeneration of protoplasts from Cryptococcus
Authors:J C Rhodes  K J Kwon-Chung
Abstract:Protoplasts were quickly and efficiently produced from both varieties of Cryptococcus neoformans and from C. laurentii by use of the multi-enzyme product Novozym 234. Conditions for regeneration of protoplasts are described. DNA yield from the Novozym-produced protoplasts was superior to that from snail gut enzyme-derived protoplasts.
Keywords:
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