Comparative studies on the diversity of the edible mushroom Pleurotus eryngii: ITS sequence analysis, RAPD fingerprinting, and physiological characteristics |
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| Authors: | Hyeon-Su Ro Sung Soon Kim Jae San Ryu Che-Ok Jeon Tae Soo Lee Hyun-Sook Lee |
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| Institution: | 1. Department of Microbiology and Research Institute of Life Science, Gyeongsang National University, 900 Gajwa-Dong, Chinju 660-701, Korea;2. Environmental Biotechnology National Core Research Center, Gyeongsang National University, 900 Gajwa-Dong, Chinju 660-701, Korea;3. Plant Environment Research Division, GyeongNam Agricultural Research and Extension Services, 1085-1 Chojeon-Dong, Chinju 660-370, Korea;4. Department of Biology, University of Incheon, 177 Dohwa-Dong, Incheon 402-749, Korea;1. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China;2. Guizhou Provincial Institute of Crop Germplasm Resources, Guiyang 550006, PR China;3. Guizhou Key Laboratory of Agricultural Biotechnology, Guiyang 550006, PR China;4. Guizhou Academy of Agricultural Sciences, Jinlong Community, Xiaohe District, Guiyang 550006, Guizhou, PR China;5. INRA, UR1264, Mycologie et Sécurité des Aliments, 33883 Villenave d’Ornon, France;6. Institute of Excellence in Fungal Research, and School of Science, Mae Fah Luang University, Tasud, Chiang Rai 57100, Thailand;7. Avda. Padre Claret 7, 5° G, 09400 Aranda de Duero, Burgos 09400, Spain;1. Instituto de Botânica, Núcleo de Pesquisa em Micologia, Caixa Postal 68041, 04045-972 São Paulo, SP, Brazil;2. Instituto Federal de Educação, Ciência e Tecnologia de São Paulo, Campus São Paulo, CCT/Biologia, Rua Pedro Vicente, 625, 01109-010 São Paulo, SP, Brazil;1. Department of Urology, Tangdu Hospital, the Fourth Military Medical University, Xi’an, China;2. Department of Radiology, Xijing Hospital, the Fourth Military Medical University, Xi’an, China;3. Department of Prosthodontics, School of Stomatology, the Fourth Military Medical University, Xi’an, China;1. Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico;2. Centro de Investigación en Nutrición y Salud, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico;3. Área Académica de Nutrición, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Pachuca, Hidalgo, Mexico;4. Unidad de Investigación Epidemiológica y en Servicios de Salud, Instituto Mexicano del Seguro Social, Cuernavaca, Mexico;5. Centro de Investigación en Salud Poblacional, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico;1. State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, Yunnan University, Kunming, 650091, Yunnan, China;2. Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China;3. University of Chinese Academy of Sciences, Beijing, 100049, China |
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| Abstract: | Verification of Pleurotus eryngii strains was assessed using ITS sequence analysis and RAPD fingerprinting. Sequence analysis of the ITS1–5.8S rDNA–ITS2 region of 24 strains of Pleurotus sp., which consisted of 22 strains of P. eryngii and the control strains P. ostreatus and P. ferulae, demonstrated that the DNA regions share mostly 99 % sequence identity, indicating that sequence-based analysis is not applicable for the verification of closely related mushroom strains. To verify the mushroom strains using RAPD, we amplified DNA fragments from the total cellular DNA of 24 mushroom strains with 18 different random primers, yielding 538 distinct DNA fragments ranging from 200–4000 bp. Analysis of the DNA fragment pattern showed that the 22 P. eryngii strains were clearly distinguished from the control strains P. ostreatus and P. ferulae, and could be categorized into five subgroups. Subsequent physiological studies on the development of fruiting bodies demonstrated the close correlation of the RAPD-based grouping with the phenotypical characteristics of mushroom fruiting bodies. |
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| Keywords: | Cultivated mushrooms Pleurotus ferulae Pleurotus ostreatus Species concepts |
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