角蛋白载药纳米颗粒的制备及药物可控释放性能研究*

目的:以角蛋白作为药物载体材料,制备智能响应性药物递送系统,研究其药物装载和释放性能。方法:利用去溶剂法制备角蛋白纳米颗粒(KNP),以罗丹明B(RB)和姜黄素(Cur)为亲水性和疏水性模式药物,制备载药KNP。利用钨灯丝扫描电镜(SEM)、动态光散射(DLS)、傅里叶变换红外光谱(FTIR)和药物体外释放实验等对KNP的尺寸、形貌、结构、载药和释药性能进行研究。结果:成功制备出粒径均一、约为300 nm 的KNP,能够装载亲水性和疏水性药物。载药颗粒在体外释放研究中表现出pH和氧化还原双重响应性。结论:利用去溶剂法,简便、安全地制备了分散性良好且具有pH和氧化还原双重响应性释放特性的角蛋白载药纳米颗粒,为角蛋白作为智能响应型药物递送载体的研究和应用提供了参考。

Preparation and Drug Release Properties of Keratin-loaded Nanoparticles

Objective: Keratin was used as the drug carrier material, and the hydrophilic drug rhodamine and hydrophobic drug curcumin were loaded to prepare the intelligent responsive drug delivery system, and the pH and redox responsiveness of drug release were studied. Methods: The exopolysaccharide of Lactobacillus plantarum was introduced into the reaction system of sodium selenite and ascorbic acid, and KNP was synthesized at room temperature. The size, morphology, structure and stability of KNP were studied by tungsten filament scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR) and drug release experiments in vitro. Results:KNP with uniform particle size of about 300 nm was successfully prepared, which can load hydrophilic and hydrophobic drugs. Keratin based nanoparticles exhibited pH and glutathione (GSH) dual responsive release characters. In addition, these drug-loaded particles showed potent prolongation duration, good stability, sustainable and controllable release than the original drug. Conclusion: KNP with high stability, good water dispersion and responsiveness can be prepared simply and safely, and the drug delivery system of keratin is an appropriate candidate drug carrier.