小麦-鹅观草第一部分同源群染色体渗入系鉴定与基因组归属分析 |
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作者姓名: | 王仪威 冯祎高 刘润然 卢春甜 曹爱忠 张瑞奇 |
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作者单位: | 南京农业大学农学院,江苏省现代作物生产协同创新中心,南京 210095 |
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基金项目: | 江苏省农业自主创新项目〔CX(19)1001〕;江苏省自然科学基金项目(BK20181316) |
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摘 要: | 鹅观草(Roegneria kamoji,2n=42,SSHHYY)是小麦异源六倍体野生近缘种,对小麦赤霉病具有良好抗性,是改良小麦赤霉病抗性的重要遗传资源。通过远缘杂交,将鹅观草第一部分同源群染色体上的抗赤霉病基因Fhb6导入普通小麦。由于第一部分同源群染色体包含1S、1H和1Y三条染色体,为研究这些同源染色体对小麦赤霉病抗性的影响,筛选出4个鹅观草第一部分同源群染色体特异分子标记,通过PCR扩增鹅观草属不同野生种的基因组DNA,明确了抗赤霉病Fhb6基因位于鹅观草1Y#1染色体。进一步利用分子细胞遗传学技术从中国春与鹅观草的后代中选育出5份涉及鹅观草1Y#2和1S#2染色体的渗入系材料。其中:21RK?1为二体异代换系DS1Y#2(1A),21RK?2为二体异代换系DS1S#2(1D),21RK?3为二体异附加系DA1S#2,21RK?4为1S#2和TW·1S#2S的双单体附加系,21RK?5为纯合TW·1S#2S易位系。这些新种质为小麦抗赤霉病基因的发掘及遗传改良奠定了基础。
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关 键 词: | 小麦赤霉病 鹅观草 Fhb6 1Y染色体 1S染色体 |
收稿时间: | 2021-06-15 |
Introgression and Characterization of the Homologous Group 1 Chromosomes from Roegneria kamoji into Common Wheat |
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Authors: | Yiwei WANG Yigao FENG Runran LIU Chuntian LU Aizhong CAO Ruiqi ZHANG |
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Institution: | Jiangsu Collaborative Innovation Center for Modern Crop Production,College of Agronomy,Nanjing Agricultural University,Nanjing 210095,China |
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Abstract: | Roegneria kamoji (2n=42, SSHHYY) is a hexaploid wild relatives of common wheat. Due to high resistance level of Fusarium head blight (FHB), R. kamoji is regarded as a potential germplasm to explore FHB?resistant genes. The FHB resistance gene Fhb6 located on the homoeologous group 1 chromosome of R. kamoji had been introduced into common wheat through hybridization. The homoeologous group 1 chromosomes of R. kamoji included 1S, 1H or 1Y chromosome. In order to understand the effect of homoeologous chromosome on resistance of FHB, four STS markers specific to homoeologous group 1 chromosomes of R. kamoji werescreened. Subsequently, these markers were used to amplify the DNA samples of the disomic substitution line DS1RK#1(1A) carrying Fhb6 and the other relatives with different genomes. Results indicated that Fhb6 was located on 1Y#1 chromosome. Five new wheat?R. kamoji introgression lines involving 1Y#2 and 1S#2 chromosomes were identified from the backcross progeny of the second hybrid between Chinese Spring and R. kamoji. Of which, 21RK?1 was the disomic substitution line DS1Y#2(1A), 21RK?2 was the disomic substitution line DS1S#2(1D), 21RK?3 was the disomic addition line DA1S#2, 21RK?4 was the double monosomic addition line of 1S#2 and TW·1S#2S, and 21RK?5 was the homologous TW·1S#2S translocation line. These new germplasm resources and the specific molecular markers developed in the present study provided the foundation for the genetic improvement of wheat resistance to Fusarium head blight. |
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Keywords: | Fusarium head blight (FHB) Roegneria kamoji Fhb6 gene chromosome 1Y chromosome 1S |
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