芍药花药愈伤组织诱导及体细胞胚发生

以芍药(Paeonia lactiflora)品种粉玉奴花药为外植体，研究不同浓度2，4-D对愈伤组织诱导、体胚发生及植株再生的影响，采用组织细胞学方法观察愈伤组织以及体细胞胚发育过程，采用根尖染色体法鉴定再生植株倍性。结果表明，芍药花药愈伤组织诱导的最适培养基为MS+1 mg·L–1 2，4-D+1 mg·L–1 N...

Callus Induction and Somatic Embryogenesis in Anther Culture of Paeonia lactiflora

The anthers of Paeonia lactiflora cv. ‘Fenyunu’ were used as explants to study the effects of different concentrations of 2,4-D on callus induction, somatic embryogenesis and plant regeneration. The cell composition of callus and the development process of somatic embryos were observed with cytohistological method, and the ploidy of regenerated plants was identified using root tip squash method. The results showed that the suitable medium for callus induction of P. lactiflora anther was MS+1 mg·L^-12,4-D+1 mg·L^-1NAA+0.1 mg·L^-1KT+30 g·L^-1sucrose+6.5 g·L^-1agar, and the callus induction rate was 14.7%. The callus was transferred to somatic embryo induction medium and underwent stages of spherical embryo, heart-shaped embryo, torpedo embryo and cotyledon embryo, and the somatic embryo induction rate was 52.1%. Genuine leaves germinated in seedling medium and complete plants were obtained, and the seedling rate was 47.1%. Haploid and diploid plants were identified using root tip squash method. The study preliminarily established a culture system to implement plant regeneration through somatic embryogenesis, which also provided reference protocol for anther culture of other varieties of Paeonia. Regenerated plants are important materials for genetic research and haploid breeding of P. lactiflora.