濒危植物百山祖冷杉种子发育特征及其胚培养

百山祖冷杉(Abies beshanzuensis)种子萌发率低，发育特性尚不明确，极大地限制了种群在原生境的自然更新。该研究以百山祖冷杉不同发育时期的雌球果为材料，通过研究球果中种子的胚与胚乳数量、重量和显微结构特性，及测定种子发育关键时期胚乳的初生代谢物，探究种子发育特征及影响种子发育的主要因素。结果表明，7月是百...

Seed Development and Embryo Culture of Endangered Abies beshanzuensis

The seed germination rate of Abies beshanzuensis is low, and the characteristics of seed development are elusive, which severely limit the natural regeneration of the population in situ. To illustrate the developmental characteris-tics of seeds and identify the main factors affecting seed development, this article aimed to study the number, weight, and microstructural characteristics of the embryo and endosperm of the seed in the female cones at different development stages, as well as the primary metabolites of the endosperm in the critical period of seed development. The results showed that July was a critical period for seed development of A. beshanzuensis. During this period, the endosperm weight increased rapidly and the carbohydrate metabolism in the endosperm was active. Anatomical and morphological analysis of the female cones showed that a large number of abnormally developed seeds appeared in cones in late July. Analysis of the primary metabolites of endosperm during the critical period showed that the content of trehalose-6-phosp-hate in the endosperm of abnormally developed seeds was significantly decreased. It was speculated that during the critical period of seed development, the decrease of trehalose-6-phosphate in the endosperm may be an important cause of abnormal seed development. Based on the characteristics of A. beshanzuensis seed development, we established the embryo culture technology for A. beshanzuensis, and generated test-tube plantlet.