苄非他明人工抗原合成

目的: 通过化学方法对苄非他明进行结构修饰并保留抗原决定簇,将结构改造后的产物与载体偶联合成苄非他明抗原。方法: 苄非他明经化学修饰后,增加活性基团连接上一类可用的经化学修饰的连接臂,使用碳二亚胺法与载体蛋白偶联成苄非他明人工合成抗原。该抗原通过紫外吸收光光谱扫描技术、SDS-PAGE电泳法及胶体金免疫层析法进行偶联效果和抗原活性的鉴定。结果: 苄非他明半抗原结构与载体偶联成功,该抗原具有较高的纯度和活性,与苄非他明抗体反应表现出较高的特异性。结论: 该方法合成的苄非他明抗原可用于免疫检测方法,也可作免疫原制备相关抗体。

Synthesis of Benzfetamine Artificial Antigen

Objective: To produce benzyphetamine antigen by coupling modified benzyphetamine using the chemical method with carrier protein and its epitope was preserved.Methods: Benzafetamine artificial antigen was prepared by conjugation of carrier protein with benzafetamine hapten that was synthesized by adding active groups to a class of available chemically modified link arms by the carbodiimide method.The results were identified by UV absorption spectroscopy, SDS-PAGE and colloidal gold immunochromatography.Results: Through these tests, the Benzfetamine from this experimental scheme was successfully coupled to the carrier.This antigen had high purity,good activity and good anti-cross interference reaction,which also had high specificity with Benzfetamine antibody.Conclusion: The Benzfetamine antigen synthesized by this method can be used in immunoassay methods, and can also be used as an immunogen to prepare relevant antibodies.