| PARP酶抑制剂诱导转化细胞逆转及外源ras癌基因丢失的研究 |
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| 引用本文： | 杨其伟,董元舒,陈雅文,陈德风YANG Qi-Wei,DONG Yuan-Shu,CHEN Ya-Wen,CHEN De-Feng.PARP酶抑制剂诱导转化细胞逆转及外源ras癌基因丢失的研究[J].遗传,1996,18(3):6-8. |
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| 作者姓名： | 杨其伟 董元舒 陈雅文 陈德风YANG Qi-Wei DONG Yuan-Shu CHEN Ya-Wen CHEN De-Feng |
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| 作者单位： | 天津南开大学分子生物学研究所, 天津 300071
Institute of Molecular Biology,Nankai University,Tianjin 300071 |
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| 摘 要： | 用 5mmol/L PARP抑制剂苯甲酰胺处理经c-Ha-T24-ras活化癌基因转染得到的转化细胞系, 处理4周后发现转化细胞系的某些细胞生物学特性发生了改变,呈现出正常细胞的某些特性。伴随这一现象,检测到细胞中整合的外源T24-ras基因发生了丢失。
Abstract:A transformant line obtained by transfection of NTH3T3 cells with c-Ha-T24-ras gene was treated with 5mmol/L benzamide,an inhibitor of PARP enzyme for 4 weeks.Some changes in cellular properties of the transformant line were observed.Concomitant with these changes was the deletion of the exogenous c-Ha-T24-ras which had been integrated into genomic DNA of recipient cells.
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| 关 键 词： | Transformed cell reversion Poly(ADP-ribosy)ation 基因丢失 Key words ras癌基因 转化细胞逆转 序列分析 古DNA 恐龙蛋 分子系统学 18S rRNA |
Studies on Reversion of a Transformant Line and Loss of Exogenous ras Oncogene after Treatment with an Inhibitor of PARP Enzyme |
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| Yang Qiwei, Dong Yuanshu, Chen Yawen ,Chen Defeng.Studies on Reversion of a Transformant Line and Loss of Exogenous ras Oncogene after Treatment with an Inhibitor of PARP Enzyme[J].Hereditas,1996,18(3):6-8. |
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| Authors: | Yang Qiwei Dong Yuanshu Chen Yawen Chen Defeng |
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| Abstract: | A transformant line obtained by transfection of NIH3T3 cells with c-Ha-T24-ras gene was treated with 5mmol / L benzamide, an inhibitor of PARP cnzyme for 4 weeks. Some changes in cellular properties of the transformant line werc obscrved. Concomitant with these changes was the deletion of the exogenous c-Ha-T24-ras which had been integrated into genomic DNA of recipient cells. |
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| Keywords: | Poly (ADP-ribosyl) ation Transformed cell reversion ras oncogene Deletion |
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