组织凝集素在人染色体上的基因定位

本文用非放射性DIG标记组织凝集素基因探针研究其在人染色体上的基因定位,首先对基因原位杂交条件进行了一系列研究。结果表明采用染色体温和变性、基因片段探针等为较好的基因原位杂交条件,通过135个细胞中期相的分析结果显示, 杂交阳性率为20%,本底为2.81,杂交点分布峰位于3q12-13。Abstract:The localization of sarcolectin gene on human chromosome is studied.A modified system of in situ hybridization was developed.A specific signal was generated by employing a nonradioactive DIG-labelled gene fragment as a probe in combination with the hybridization buffer containing no dextran sulphate and softly denatured chromosomes.Using this system,unique sequence for sarcolectin as small as 0.65kb was detectable and observed at 3q12-13.Of 135 metaphases examined,27(20%) had at least one grain deposited on 3q12-13,and the backgrand was 2.81.

Localization of Sarcolectin Gene on Human Chromosome

The localization of sarcolectin gene on human chromosome is studied. A modifled system of in situ hybridization was developed. A specific signal was generated by cmploying a nonradioactive DIG-labelled gene fragment as a probe in combination with the hybridisation buffer containing no dextran sulphate and softly denatured chromosomes. Using this system, unique sequcnce for sarcolectin assmall as 0.65kb was detectable and observed at 3q 12-13. Of 135 metaphases examined, 27(20%) had at least one grain deposited on 3q 12-13, and the backgrand was 2.81.