Gel-phase synthesis of a difficult sequence peptide on 1,4-butanediol dimethacrylate-crosslinked polystyrene support |
| |
Authors: | Krishnakumar I M Mathew Beena |
| |
Institution: | (1) School of Chemical Sciences, Mahatma Gandhi University, Priyadarshini Hills, Kottayam, Kerala, India |
| |
Abstract: | Summary The synthetic usefulness of the protocol using NMP/DMSO and DIEA for the synthesis of difficult sequence peptides on amphiphilic
and flexible 1,4-butanediol dimethacrylate-crosslinked polystyrene (BDDMA-PS) support was demonstrated by synthesizing DAla17] analogue of gonadotropin releasing hormone precursor protein fragment (14–36) hGnRH (14–36)] using Boc chemistry. The swelling
capacity of the peptidyl resin was followed as a measure of the aggregation of pendant peptide chains on the support. The
progress of chain assembly was monitored by quantitative ninhydrin test and amino acid analysis. The purity of the peptide
was checked by reverse phase HPLC and characterized by amino acid analysis and electrospray ionisation mass spectrometry (ESI-MS). |
| |
Keywords: | aggregation β -sheet structure coupling difficulty difficult sequences solid-phase peptide synthesis |
本文献已被 SpringerLink 等数据库收录! |