Non-cognizable ribonucleotide, 2'AMP, binds to a mutant ribonuclease T1 (Y45W) at a new base-binding site but not at the guanine-recognition site. |
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Authors: | T Hakoshima T Itoh K Gohda K Tomita S Uesugi S Nishikawa H Morioka E Ohtsuka M Ikehara |
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Affiliation: | Faculty of Pharmaceutical Sciences, Osaka University, Japan. |
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Abstract: | Complex of a mutant ribonuclease T1 (Y4SW) with a non-cognizable ribonucleotide, 2′AMP, has been determined and refined by X-ray diffraction at 1.7 Å resolution. The 2′AMP molecule locates at a new base-binding site which is remote from the guanine-recognition site, where 2′GMP was found to be bound. The nucleotide adopts the anti conformation of the glycosidic bond and C3′-exo sugar pucker. There exists a single hydrogen bond between the adenine base and the enzyme, and, therefore, the site found is apparently a non-specific binding site. The results indicate that the binding of 2′AMP to the guanine-recognition site is weaker than that to the new binding site. |
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Keywords: | X-ray Recognition Non-specific binding Mutant RNase T1 |
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