| 冬小麦原生质体培养的胚状体直接发生 |
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| 引用本文： | 葛台明.冬小麦原生质体培养的胚状体直接发生[J].生物工程学报,2000,16(5):609-613. |
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| 作者姓名： | 葛台明 |
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| 作者单位： | 1. 湖北民族学院园艺系,思施,445000
2. 华中农业大学作物遗传改良国家重点实验室,武汉,430070 |
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| 基金项目： | 华中农业大学作物遗传改良国家重点实验室开放项目资助,湖北省教委青年项目提供了部分资助。 |
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| 摘 要： | 冬小麦品种“京花一号”胚性愈伤组织在改良的N6培养基(NBD培养基)上继代得到易碎型胚性愈伤组织,转入改良MS液体培养基(MSDL培养基)后得到胚性悬浮系,分离的原生质体在改良的MS培养基(MSDP培养基)上培养,再生细胞直接产生体细胞胚胎,并再生出完整植株。体细胞胚胎形成过程与小麦合子胚的形成过程十分相似。
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| 关 键 词： | 冬小麦 京花一号 原生质体培养 体细胞胚胎直接发生 植株再生 |
| 文章编号： | 1000-3061-(2000)05-0609-05 |
| 修稿时间： | 1999-11-17 |
Direct Embryogenesis from Protoplast of Winter Wheat |
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| GE Tai-Ming,ZHANG Rong-De,QIN Fa-Lan,YU Yu-Jun,XIE Yue-Feng.Direct Embryogenesis from Protoplast of Winter Wheat[J].Chinese Journal of Biotechnology,2000,16(5):609-613. |
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| Authors: | GE Tai-Ming ZHANG Rong-De QIN Fa-Lan YU Yu-Jun XIE Yue-Feng |
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| Institution: | GE Tai-Ming
;(Department of Horticulture, Hubei Institute for Nationalities, Enshi 445000);ZHANG Rong-De
,QIN Fa-Lan
,YU Yu-Jun
,XIE Yue-Feng
;(National Key Laboratory of Crop Crentic Improvement, Huazhong Agricultural University, Wuhan 430070) |
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| Abstract: | Friable embryogenic calli were obtained on a modified N medium (NBD medium) from a winter wheat cultivar "Jinghua No.1"(Triticum aestivurn L. cu. Jinghua No.1) and were transferred to a modified MS liquid medium (MSDL medium) to initiate embryogenic suspension cultures. Protoplasts were isolated from the suspensions and cultured on a modified MS medium (MSDP medium). The somatic embryoids were formed directly from the protoplasts and germinated into entire plants. The development of the somatic embryoids was very similar to that of zygotic embryos of wheat. |
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| Keywords: | Winter wheat (Triticum aestivum L ) Jinghua No 1 protoplast culture direct somatic embryogenesis plant regeneration |
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