Cholesteryl oligoethyleneglycol glycosides: fluidizing effect of their embedment into phospholipid bilayers |
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Authors: | Bardonnet Pierre-Louis Faivre Vincent Pirot Fabrice Boullanger Paul Falson Françoise |
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Affiliation: | Laboratoire de Pharmacie Galénique Industrielle, EA 3741, ISPB, Université Claude Bernard, Lyon I, 8 av. Rockefeller, 69373 Lyon, France. |
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Abstract: | Glycosides of cholesteryl oligoethyleneglycols have been synthesized and embedded in liposome bilayers. Several methods as steady-state fluorescence polarization, differential scanning calorimetry, zeta potential, and agglutination have been used to describe the physicochemical outcome of the incorporation of these synthetic glycolipids within phospholipid layers. From calorimetry and fluorescence experiments, it is apparent that the glycolipids decrease the transition temperature of the bilayers in a more important extent than cholesterol. Furthermore, the thickness of the aqueous layer fixed around the liposome is independent of the presence of glycolipids, suggesting that their hydrophilic parts (tetraethyleneglycol spacer and sugar moiety) are not completely extended towards the aqueous phase. Nevertheless, an important and specific interaction has been observed between such glycosylated liposomes and vegetal lectins. |
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Keywords: | Glycolipid Liposome Phospholipid Cholesterol Differential scanning calorimetry Fluorescence Zeta potential Agglutination Lectin |
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