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| 巴西橡胶树水通道蛋白基因cDNA的克隆及序列分析 | |
| 引用本文： | 庄海燕,安锋,何哲,丁璇,吴海平,徐智娟,张硕新.巴西橡胶树水通道蛋白基因cDNA的克隆及序列分析[J].西北植物学报,2010,30(5). |
| 作者姓名： | 庄海燕 安锋 何哲 丁璇 吴海平 徐智娟 张硕新 |
| 作者单位： | 1. 西北农林科技大学,林学院,陕西杨陵,712100;农业部橡胶树生物学重点开放实验室,海南儋州,571737 2. 农业部橡胶树生物学重点开放实验室,海南儋州,571737 3. 西北农林科技大学,林学院,陕西杨陵,712100 |
| 基金项目： | 国家自然科学基金，国家自然科学基金国际合作项目，公益性行业(农业)科技专项，海南省自然科学基金 |
| 摘 要： | 采用简并性RT-PCR和RACE技术,从巴西橡胶树(Hevea brasiliensis)中克隆了2个aquaporin基因的全长cDNA,分别命名为HbPIP1和HbPIP2(GenBank登录号分别为GQ479823和GQ479824).测序和生物信息学分析表明,HbPIP1和HbPIP2分别编码287和278个氨基酸,均具有6个跨膜区,包含MIP家族信号序列SGX-HXNPAVT/SFG、高等植物PIP高度保守序列GGGANXXXXGY和TGI/TNPARSL/FGAAI/VI/VF/YN.核酸和氨基酸序列的同源性分析表明,HbPIP1和HbPIP2均属于水通道蛋白基因家族的新成员. |
| 关 键 词： | 巴西橡胶树 水通道蛋白 序列比对 |
Molecular Cloning and Sequence Analysis of cDNAs Encoding Aquaporins in Hevea brasiliensis |
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| ZHUANG Hai-yan,AN Feng,HE Zhe,DING Xuan,WU Hai-ping,XU Zhi-juan,ZHANG Shuo-xin.Molecular Cloning and Sequence Analysis of cDNAs Encoding Aquaporins in Hevea brasiliensis[J].Acta Botanica Boreali-Occidentalia Sinica,2010,30(5). | |
| Authors: | ZHUANG Hai-yan AN Feng HE Zhe DING Xuan WU Hai-ping XU Zhi-juan ZHANG Shuo-xin |
| Institution: | ZHUANG Hai-yan1,2,AN Feng2,HE Zhe2,DING Xuan2,WU Hai-ping2,XU Zhi-juan2,ZHANG Shuo-xin1(1 College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China,2 Key Laboratory of Rubber Biology of Ministry of Agriculture,Danzhou,Hainan 571737,China) |
| Abstract: | By the technology of RT-PCR and RACE,two full-length cDNAs encoding aquaporins were cloned from Hevea brasiliensis,named HbPIP1 and HbPIP2.The accession numbers were GQ479823 and GQ479824 in GenBank.Bioinformatics software analysis shows that,the two aquaporin genes HbPIP1 and HbPIP2 encode 287 amino acids and 278 amino acids,respectively.HbPIP1 and HbPIP2 both exhibit six transmembrane domains,possessing the MIP family signal consensus sequence-SGXHXNPAVT/SFG,the highly conservative sequence-GGGANXXXXGY,TG... |
| Keywords: | RT-PCR RACE |
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