Pteridines are produced during interleukin 2-induced T-cell proliferation and modulate transmission of this signal |
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| Authors: | Irmgard Ziegler Udo Schwulera Joachim Ellwart |
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| Institution: | 1. Department of Chemical Engineering and Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India;2. Department of Physics & Astrophysics, University of Delhi, Delhi 110007, India;3. NanoBioelectronics Laboratory, Department of Biotechnology, Delhi Technological University, Main Bawana Road, Delhi 110042, India;4. ERA Chair of Green Chemistry, Department of Chemistry and Biotechnology, Tallinn University of Technology, 12618 Tallinn, Estonia;5. Department of Biological Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences (Formerly Allahabad Agricultural Institute), Allahabad 221007, Uttar Pradesh, India;6. Department of Biochemistry and Immunology, Ribeirao Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil;7. Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak 124001, Haryana, India;8. Microbial Process Development Laboratory, Department of Biotechnology, Central University of Haryana-Mahendergarh, 123031, India |
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| Abstract: | Pteridine levels of interleukin 2 (IL-2) receptor+ T-cell populations have been determined by HPLC after iodine oxidation; neopterin was monitored in the culture supernatants by radio-immunoassay. Upon addition of IL-2, cellular levels of biopterin and 6-hydroxymethylpterin rise transiently from 0.02 to 0.9 pmol/10(6) cells, cellular levels of neopterin from 1.5 to 4.1 pmol/10(6) cells. They peak at 8 and 13 h, respectively, after exposure to IL-2. Neopterin is not accumulated in the culture supernatant. DNA synthesis in T cells begins 10-12 h after adding the lymphokine and the portion of cells that undergo S-phase transition gradually increases during the subsequent 10 h. Entry into DNA synthesis phase is markedly accelerated if IL-2 is supplied together with tetrahydrobiopterin (0.8-1.6 X 10(-6) M) and the kinetics of entry into the S-phase transition during the period of 6-20 h become linear. This indicates that tetrahydrobiopterin modulation of IL-2 activity (Ziegler, I. et al. Naturwiss 72 (1985) 330) is an early event occurring during IL-2 signal transmission. |
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