Immortalization of rat kidney glomerular mesangial cell and its coculture with glomerular epithelial cell |
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Authors: | Toshinobu Kida Sachi Fujishima Masatoshi Matsumura Pi-Chao Wang |
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Institution: | (1) Institute of Applied Biochemistry, Tsukuba University, 1-1-1 Tennodai, 305-8572 Tsukuba City, Japan |
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Abstract: | Mesangial cell has several key roles in the control of glomerular function: it participates in the regulation of glomerular
filtration rate, macromolecular clearance, and as both a source and target of numerous hormones and autocrines. Many of these
insights into mesangial cell function have been obtained by studying mesangial cells in culture. However, no suitable cell
lines have been established yet. We here reported the immortalization of rat kidney glomerular mesangial cell by transfection
of E6 and E7 genes of human papillomavirus type 16 (HPV-16) via electroporation and lipofection. The results showed that only
electroporation could transfect the genes to mesangial cells and the transfected cells maintained the viability for longer
than 6 months. Fluorescence microscopic observation showed that cellular contractility and phagocytosis, which are the two
main phenotypes of mesangial cells, are well maintained after transfection. The coculture of transfected mesangial cells with
rat glomerular epithelial cells showed that the growth of mesangial cells was suppressed by epithelial cell, but the growth
of epithelial cells was enhanced by mesangial cells. Moreover, an enhancing effect on the phagocytosis of mesangial cell was
also observed in coculture. Such results may imply that the glomerular cell-cell interaction plays an important role in the
regulation of cell proliferation and differentiation. |
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Keywords: | immortalization glomerular mesangial cell glomerular epithelial cell E6 and E7 genes cellular contractility phagocytosis |
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