Assay-dependent effect of silver nanoparticles to <Emphasis Type="Italic">Escherichia coli</Emphasis> and <Emphasis Type="Italic">Bacillus subtilis</Emphasis> |
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Authors: | Shin Woong Kim Yong-Wook Baek Youn-Joo An |
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Institution: | (1) Department of Environmental Science, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Korea; |
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Abstract: | We assess the microbial assay-dependent effect of AgNP on gram-negative Escherichia coli and gram-positive Bacillus subtilis. The experiment was conducted via three different assays: a growth inhibition assay, a colony forming unit assay, and a liquid-to-plate
assay. AgNP were exposed either as liquid suspensions or in an agar state. Bacterial sensitivity to AgNP was found to be dependent
on the microbial assay employed. E. coli was more sensitive than B. subtilis in the growth inhibition and CFU assays, but B. subtilis was more vulnerable than E. coli in the liquid-to-plate assay, ostensibly owing to the food stress mechanisms of B. subtilis in exposure medium. The dissolution of silver from AgNP could not explain the observed toxicity of AgNP. We detected clear
evidence of AgNP uptake by cells. The results of this study showed that the microbial toxicity of AgNP and the effects of
dissolved silver ions were influenced profoundly by the microbial test method employed. |
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