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| 长双歧杆菌NCC2705群体感应系统信号分子AI-2的检测 | |
| 引用本文： | 姜铮,陈宣男,王雪松,刘大伟,郭燕红,赵江丽,邵长林,袁静,何湘. 长双歧杆菌NCC2705群体感应系统信号分子AI-2的检测[J]. 生物技术通讯, 2010, 21(4): 540-543. DOI: 10.3969/j.issn.1009-0002.2010.04.020 |
| 作者姓名： | 姜铮 陈宣男 王雪松 刘大伟 郭燕红 赵江丽 邵长林 袁静 何湘 |
| 作者单位： | 1. 解放军疾病预防控制所,北京,100071 2. 吉林大学,公共卫生学院,吉林,长春,130021 3. 江南大学,生物工程学院,江苏,无锡,214122 |
| 摘 要： | 目的:用生物学方法检测长双歧杆菌NCC2705是否产生群体感应系统信号分子AI-2。方法:将长双歧杆菌NCC2705不同时间点的培养上清分别加至AI-2特异报告系统哈氏弧菌BB170中,以空白培养基上清为对照,用荧光光度计对哈氏弧菌发光强度进行计量,推测出长双歧杆菌NCC2705上清中是否含有分泌的AI-2,并由此推断AI-2的活性。结果:通过微孔板检测系统对加入长双歧杆菌NCC2705培养上清的哈氏弧菌BB170进行检测,发现双歧杆菌上清的加入增强了哈氏弧菌BB170发出的荧光强度。结论:长双歧杆菌NCC2705中存在依赖于luxS/AI-2的群体感应系统,并能够分泌有活性的AI-2,为进一步研究长双歧杆菌NCC2705AI-2及luxS基因的功能打下基础。 |
| 关 键 词： | 群体感应 信号分子AI-2 长双歧杆菌NCC2705 哈氏弧菌BB170 生物发光分析 |
Detection of the Quorum-Sensing Signal Autoinducer-2 of Bifidobacterium Longum NCC2705 |
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| JIANG Zheng,CHEN Xuan-Nan,WANG Xue-Song,LIU Da-Wei,GUO Yan-Hong,ZHAO Jiang-Li,SHAO Chang-Lin,YUAN Jing,HE Xiang. Detection of the Quorum-Sensing Signal Autoinducer-2 of Bifidobacterium Longum NCC2705[J]. Letters in Biotechnology, 2010, 21(4): 540-543. DOI: 10.3969/j.issn.1009-0002.2010.04.020 | |
| Authors: | JIANG Zheng CHEN Xuan-Nan WANG Xue-Song LIU Da-Wei GUO Yan-Hong ZHAO Jiang-Li SHAO Chang-Lin YUAN Jing HE Xiang |
| Affiliation: | 1. Institute of Disease Control and Prevention, Academy of Military Medical Science, Beijing 100071; 2. School of Public Health, Jilin University, Changchun 130021; 3. School of Biotethnology, Jiangnan University, Wuxi 214036; China |
| Abstract: | Objective： To explore that if the Bifidobacterium longum NCC2705 produced the quorum-sensing signal autoinducer-2（AI-2） by utilizing a biological assay. Methods： The experiment employs a bioluminescent bacterial reporter strain called Vibrio harveyi BB170, which produces light in response to AI-2. We collected the cellfree culture fluids and add them to the V.harveyi reporter strain. The resulting light production was measured using a luminometer or scintillation counter. AI-2 activity was deduced according to the induction of luminescence of V. harveyi BB170. Results： The fluorescence of the V.harveyi increased after adding the cell-free culture fluids of B. logum NCC2705. Conclusion： We find that the B.logum produces the quorum-sensing signal AI-2, laying the foundation to further study of function of AI-2 from B.logum. |
| Keywords: | quorum-sensing autoinducer-2 Bifidobacterium longum NCC2705 Vibrio harveyi BB170 bioluminescent assay |
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