水稻恢复系M630稻瘟病抗性改良及其代谢组研究

稻瘟病是水稻的主要病害之一,改良水稻稻瘟病抗性对水稻生产、推广具有重要意义.本研究以携带广谱抗稻瘟病基因Pi9材料9311-Pi9为供体,水稻优良恢复系M630为受体,将杂交、回交与分子标记辅助选择和背景筛选相结合,获得改良恢复系M630-Pi9.主要农艺性状和稻米品质分析显示改良后的M630-Pi9及其杂交组合徽两优...

Genetic Improvement of Rice Blast Resistance in Restorer Line M630 and Metabolomic Analysis

Rice blast is one of the main diseases in rice,and improvement of rice blast resistance is of great significance in rice breeding.In this study,we constructed a new restorer line M630-Pi9 by making crosses in the rice variety 9311-Pi9 that carries the broad-spectrum blast resistance gene Pi9 with the excellent rice restorer line M630,followed by backcrossing combined with molecular marker-assisted selection and background detection.No significant difference on agronomic traits and rice quality was observed in M630-Pi9 and its hybrid Huiliangyou 630-Pi9 if relative to M630 and Huiliangyou 630,respectively.By test for rice blast resistance at the seeding stage was conducted in M630-Pi9 and M630 by using a mixture of predominant isolates from Anhui province,M630-Pi9 represented improved resistance than that of M630.Natural inoculation showed that the improved restorer lines and derived hybrids had obviously improved rice blast resistance.Moreover,a metabolomic analysis was conducted in order to identify the metabolites involved in rice blast resistance.212 metabolites in M630-Pi9 compared with M630 were found,including 155 down-regulated and 57 up-regulated components.The contents of substances annotated for cell wall and alkaloids which have toxic or immunizing effects on the organism are obviously increased and decreased,respectively.The contents of flavonoids involved in adverse biological process have been modified,implying that the rice blast resistance gene Pi9 trade off the invasion of pathogens via possibly regulating the contents of metabolites.Collectively,this work provided an example of deciphering the mechanism of rice blast resistance and producing new germplasm for rice breeding.