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紫色红曲霉对沙棘青稞复合酵素性能的影响研究
引用本文:朱雪洋,陶瑾,张莉方,徐宁莉,张国强.紫色红曲霉对沙棘青稞复合酵素性能的影响研究[J].微生物学报,2022,62(12):4825-4840.
作者姓名:朱雪洋  陶瑾  张莉方  徐宁莉  张国强
作者单位:安徽工程大学生物与食品工程学院, 安徽 芜湖 241000
基金项目:安徽省重点研发计划项目(201904e01020008);西藏自治区重点研发项目(XZ202001ZY0040N);安徽工程大学中青年拔尖人才项目(2018–2019)
摘    要:【目的】为了对比分析紫色红曲霉是否对沙棘青稞酵素具有促进作用。【方法】以对照组、沙棘组、沙青组、沙青红曲组4种发酵液为研究对象,对其pH、总糖、总酸、可溶性固形物、总酚、总黄酮、抗坏血酸和洛伐他汀、超氧化物歧化酶、脂肪酶和蛋白酶含量、1,1-二苯基-2-苦苯肼自由基清除能力、2,2ʹ-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基清除能力、酵母菌活菌数和乳酸菌活菌数进行比较分析。【结果】沙青红曲组的总酚、总黄酮、抗坏血酸和洛伐他汀、超氧化物歧化酶、脂肪酶和蛋白酶含量、1,1-二苯基-2-苦苯肼自由基清除率、2,2ʹ-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基清除率、酵母菌活菌数和乳酸菌活菌数均显著高于沙棘组和沙青组(P<0.05)。【结论】该研究证明了紫色红曲霉的添加可提高酵素的性能,对酵素行业的多元化发展有潜在的促进作用。

关 键 词:紫色红曲霉  生物活性物质  功能酶活力  抗氧化活性  微生物活菌数
收稿时间:2022/3/27 0:00:00
修稿时间:2022/6/10 0:00:00

Effect of Monascus purpureus on the characteristics of sea buckthorn-highland barley complex Jiaosu
ZHU Xueyang,TAO Jin,ZHANG Lifang,XU Ningli,ZHANG Guoqiang.Effect of Monascus purpureus on the characteristics of sea buckthorn-highland barley complex Jiaosu[J].Acta Microbiologica Sinica,2022,62(12):4825-4840.
Authors:ZHU Xueyang  TAO Jin  ZHANG Lifang  XU Ningli  ZHANG Guoqiang
Institution:College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, Anhui, China
Abstract:Objective] To analyze whether Monascus purpureus can improve the characteristics of sea buckthorn-highland barley complex Jiaosu. Methods] The pH, total sugar, total acids, soluble solids, total phenols, total flavonoids, ascorbic acid and monacolin K, superoxide dismutase, lipase, protease, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging rate, 2,2ʹ-azino-bis(3-ethylbenzothiazoline- 6-sulfonic acid (ABTS) free radical scavenging rate, number of active yeast, and number of active lactobacilli were compared between control group, sea buckthorn group, sea buckthorn-highland barley Jiaosu group, and sea buckthorn-highland barley Jiaosu+M. purpureus group. Results] The sea buckthorn-highland barley Jiaosu+M. purpureus group had higher total phenols, total flavonoids, ascorbic acid and monacolin K, superoxide dismutase, lipase, protease, DPPH free radical scavenging rate, ABTS free radical scavenging rate, number of active yeast, and number of active lactobacilli than the sea buckthorn group and the sea buckthorn-highland barley Jiaosu group (P<0.05). Conclusion] The addition of M. purpureus can improve the characteristics of Jiaosu and plays a potential role in promoting the diversified development of the Jiaosu industry.
Keywords:Monascus purpureus  bioactive substances  functional enzyme activity  antioxidant activity  live microbial count
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