玉米可溶性无机焦磷酸酶家族基因的克隆和表达分析

可溶性无机焦磷酸酶(Soluble inorganic pyrophosphatase)通过水解作用调节细胞质中无机焦磷酸盐(PPi,pyrophosphate)的含量使其维持在适当水平.本研究一共克隆了玉米(Zea mays L.)中7个可溶性无机焦磷酸酶基因ZmPPases,分别位于第2、4、5、6、8、10号染色...

Cloning and Expression Analysis of Soluble Inorganic Pyrophosphatase Family Genes in Maize(Zea mays L.)

Soluble inorganic pyrophosphatase regulates the homeostasis of inorganic pyrophosphate(PPi)in the cytoplasm through hydrolysis.In this study,seven genes encoded for soluble inorganic pyrophosphatase in maize(ZmPPases)were cloned and identified on chromosomes 2,4,5,6,8,10,respectively.The relative molecular mass of ZmPPases was varied from 22.8 kDa to 25.6 kDa with the isoelectric point of 4.84 to 6.24.Secondary structure analysis of ZmPPases revealed alpha helix and beta strand motifs.Phylogenetic analysis together with the homozygous proteins in maize,rice,Sorghum,and Arabidopsis suggested three phylogenetic branches of soluble inorganic pyrophosphatases.ZmPPases were localized in the cell membrane and nucleus by transient expression in Nicotiana benthamiana L..The ZmPPase genes were inducible under salt and drought stress treatments,whereas ZmPPase2.1(Zm00001 d051564)was down-regulated under salt stress treatment.Collectively,this study reported a comprehensive analysis of the sequence and expression of ZmPPases family members,which might provide a theoretical basis for further functional research.